Primary cells that reach the end of their replicative potential, encounter sublethal stress, or experience the
activation of certain oncogenes cease proliferation and enter a state of long-term growth arrest named
senescence. The senescent process has been implicated in a variety of age-related diseases and also in the
pathogenesis of cancer. Senescence is characterized by distinct changes in the types and levels of coding
RNAs (mRNAs) as well as in the vast collective of regulatory noncoding (nc)RNAs, which includes
microRNAs, long noncoding RNAs (lncRNAs), and circular (circRNAs). Numerous technologies permit
the detection of senescence-associated linear transcripts (mRNAs, lncRNAs, microRNAs), but the identifi cation
and quantifi cation of circRNAs in senescence require distinct molecular approaches. In this chapter,
we describe a method for the detection and measurement of circRNAs in senescent cells using specialized
reverse transcription (RT) followed by real-time quantitative (q)PCR analysis.

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